Spearmint (Mentha spicata L.) essential oil from tipaza (Algeria): in vivo anti-inflammatory and analgesic activities in experimental animal models

Authors

  • Sarah Kehili Laboratory of Research on Bio-Active Products and Valorization of Biomasse, Department of Chemistry, Ecole Normale Superieure, Algiers, Algeria
  • Mohamed Amine Boukhatem Department of Biology and Cell Physiology, Faculty of Life and Natural Sciences, University - Saad Dahab - Blida 1, Blida, Algeria
  • Asma Belkadi Laboratory of Pharmacology and Toxicology, Research and Development Center, SAIDAL Pharmaceuticals, Algiers, Algeria
  • Faiza Boulaghmen Anatomic Pathology Laboratory, Hospital - Dr Fares Yahia - Kolea, Tipaza, Algeria
  • Mohamed Amine Ferhat Laboratory of Research on Bio-Active Products and Valorization of Biomasse, Department of Chemistry, Ecole Normale Superieure, Algiers, Algeria
  • William N. Setzer Department of Chemistry, University of Alabama in Huntsville, Huntsville, USA Aromatic Plant Research Center, Lehi, USA

DOI:

https://doi.org/10.33892/aph.2020.90.15-26

Keywords:

Spearmint essential oil, Anti-inflammatory effect, Carvone, Histological analysis, Pharmacognosy

Abstract

Introduction

Although analgesic and nonsteroidal anti-inflammatory drugs (NSAIDs) are usually used to treat a diversity of illnesses, their administration is linked with acute kidney injury and gastrointestinal side effects. The research of new biomolecules and natural products is still needed such as medicinal plants.

Aims

The present research was aimed to investigate, for the first time, the anti-inflammatory and anti-nociceptive effects of spearmint essential oil (SEO) in mouse models of acute inflammation and pain.

Materials and Method

Chemical analysis of SEO was done by gas chromatography. The anti-inflammatory activity was tested using two models of acute inflammation namely carrageenan-induced paw edema and xylene-induced ear edema. Histological examination of both non-inflamed and inflamed tissues was evaluated. The anti-nociceptive activity was tested using the pain model induced by acetic acid.

Results

The main constituent of the SEO was found to be carvone (52.60%). The SEO exhibited a promising anti-inflammatory effect as demonstrated by statistically significant (p<0.05) inhibition of paw volume by 77.24% at the dose of 20 µL/kg and 65.87% at the dose of 200 µL/kg. Furthermore, topical administration of the SEO inhibited xylene-induced ear edema in comparison with the control group (p<0.05). The higher dose (200 µL /kg) significantly (p<0.001) reduced xylene-induced ear edema which was similar to that observed with positive control (ketoprofen). The pathological analysis of the paws and ears revealed that SEO was capable of reducing cellular infiltration and subcutaneous edema. Else, the SEO produced significant anti-nociceptive activity (p<0.001) at higher dose by inhibiting spontaneous nociception.

Conclusion

These results support the use of SEO in the development of pharmaceuticals for the management of inflammation and pain.

Published

2020-06-10

How to Cite

(1)
Kehili, S.; Boukhatem, M. A.; Belkadi, A.; Boulaghmen, F.; Ferhat, M. A.; Setzer, W. N. Spearmint (Mentha Spicata L.) Essential Oil from Tipaza (Algeria): In Vivo Anti-Inflammatory and Analgesic Activities in Experimental Animal Models. Acta Pharm Hung 2020, 90, 15-26.

Issue

Vol. 90 No. 1 (2020)

Section

Original Research Articles

Copyright (c) 2020 Acta Pharmaceutica Hungarica

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