Evaluation of the permeability and in vitro cytotoxicity of functionalized titanate nanotubes on Caco-2 cell line

Authors

  • Yasmin Ranjous Institute of Pharmaceutical Technology and Regulatory Affairs, University of Szeged, Eötvös Str. 6, 6720 Szeged, Hungary
  • Dóra Kósa University of Debrecen, Department of Pharmaceutical Technology
  • Zoltán Ujhelyi Department of Pharmaceutical Technology, University of Debrecen, Nagyerdei Körút 98, 4032 Debrecen, Hungary
  • Géza Regdon jr. Institute of Pharmaceutical Technology and Regulatory Affairs, University of Szeged, Eötvös Str. 6, 6720 Szeged, Hungary
  • Krisztina Anita Nagy Department of Applied and Environmental Chemistry, University of Szeged, Rerrich tér 1, Szeged 6720, Hungary
  • Imre Szenti Department of Applied and Environmental Chemistry, University of Szeged, Rerrich tér 1, Szeged 6720, Hungary
  • Zoltán Kónya Department of Applied and Environmental Chemistry, University of Szeged; MTA-SZTE Reaction Kinetics and Surface Chemistry Research Group, Rerrich tér 1, Szeged 6720, Hungary
  • Ildikó Bácskay Department of Pharmaceutical Technology, University of Debrecen, Nagyerdei Körút 98, 4032 Debrecen, Hungary
  • Tamás Sovány Institute of Pharmaceutical Technology and Regulatory Affairs, University of Szeged, Eötvös Str. 6, 6720 Szeged, Hungary

DOI:

https://doi.org/10.33892/aph.2021.91.31-39

Keywords:

cytotoxicity; permeability; functionalization; titanate nanotubes; magnesium stearate; silane derivatives

Abstract

Titanate nanotubes (TNTs) are promising vectors for drug delivery due to their unique physicochemical properties such as biocompatibility, mechanical strength, and chemical resistivity. However, considering their strong hydrophilicity, pristine TNTs exert very limited permeability through the intestinal cell layer. The aim of this study was to turn the surface characteristics and thus enhance the permeability of TNTs by functionalization. TNTs were functionalized with trichloro(octyl)silane (TCOS) and magnesium stearate (MgSt). Carbon content and surface free energy of the functionalized TNTs were detected to evaluate the effectiveness of functionalization, by using CHNS analytical and optical contact angle (OCA) measurements, respectively. Caco-2 cell line was applied to test the permeability and the cytotoxicity of the samples. Cytotoxicity was evaluated by using MTT assay. The results revealed that the surface characteristics of TNTs may be adjusted in a wider range with TCOS-TNT than with St, but the samples show higher toxicity. Silane functionalized TNTs may be safe up to 1 mg/ml, while St functionalized TNTs up to 2 mg/ml concentration. The preparation method of MgSt-TNT was also superior from the aspect of environmental safety. The permeability was suitable for samples with moderate hydrophobicity (aqueous contact angle 60-90°).

Published

2021-06-15

How to Cite

(1)
Ranjous, Y.; Kósa, D.; Ujhelyi, Z.; Regdon jr., G.; Nagy, K. A.; Szenti, I.; Kónya, Z.; Bácskay, I.; Sovány, T. Evaluation of the Permeability and in Vitro Cytotoxicity of Functionalized Titanate Nanotubes on Caco-2 Cell Line. Acta Pharm Hung 2021, 91, 31-39.

Issue

Section

Original Research Articles